Study of SARS-nCoV2 Indian Isolates Gaining Insights into Mutation Fre-quencies, Protein Stability and Prospective Effect on Pathogenicity

Background: SARS-nCOV-2 is a variant of the known SARS coronavirus family. The mutations in viruses are very rapid and can play a crucial role in the evolution or devolution of the organism. This has a direct impact on "host jumping" and the pathogenicity of the virus. Objective(s): The study aims to understand the frequency of genomic variations that have occurred in the virus affecting the Indian sub-population. The impact of variations translating to proteins and its consequences affecting protein stability and interaction were studied. Method(s): Phylogenetic analysis of the 140 genomes from the India region was performed, followed by SNP and Indel analysis of both CDS and non-CDS regions. This effort was followed by a prediction of mutations occurring in 8 proteins of interest and the impact on protein stability and prospective drug interactions. Result(s): Genomes showed variability in origin, and major branches can be mapped to the 2002 outbreak of SARS. The mutation frequency in CDS regions showed that 241 C >T, 3037 C >T, 2836 C >T, and 6312 C >A occurred in 81.5% of genomes mapping to major genes. Corresponding mutations were mapped to protein sequences. The effect of mutations occurring in spike glycoprotein, RNA dependent RNA polymerase, nsp8, nucleocapsid and 3c protease was also depicted. Conclusion(s): Whilst the mutations in spike glycoprotein showcased an increase in protein stability, the residues undergoing mutations were also a part of drug binding pockets for hydroxychloro-quine. Mutations occurring in other proteins of interest led to a decrease in protein stability. The mutations were also a part of drug binding pockets for Favipiravir, Remdesivir and Dexametha-sone. The work allows analyzing larger datasets to understand mutation patterns globally.Copyright © 2021 Bentham Science Publishers.

Impact of community outreach programme on improving cancer related preventive health behaviour : Vikram Niranjan

Cancer services were negatively affected due to the Covid-19 pandemic and will have likely delayed early detection and diagnosis. The Irish Cancer Society (ICS) planned and delivered a number of cancer roadshow events in 4 deprived regions in Ireland to address improvements in awareness of cancer signs, importance of screening, positive lifestyle changes and encourage medical care-seeking behaviour. Health checks (blood pressure, BMI & CO2 monitoring) and motivational interviews were carried out by ICS nurses and trained ICS volunteers. Data was collected via anonymous questionnaire from participants and volunteers. SPSS was used for analysis. 98 people (54 male, 44 female) participated;87.7% found the information provided useful, 84.7% approved of the health check and 72.5% stated that they would likely make changes to their current lifestyle to reduce their cancer risk. Moderate/high understanding of cancer signs and symptoms and moderate/high awareness of cancer risk factors both rose post event (from 62.2% to 81.6% (p < 0.001) and from 49% to 61.2% (p < 0.001) respectively). If symptomatic for cancer 77.6% of participants would likely visit their GP and 73.5% would likely contact ICS. Younger people (aged ≤40 years) were more likely to consider consulting a healthcare professional if symptomatic (p = 0.027) and to contact the ICS (p = 0.007) for more information. High numbers of participants (98.9%) and volunteers (95.2%) recommended a nationwide roll out. Volunteers reported moderate/high levels of public engagement;the most common topics discussed were own treatment experience, cancer screening, information on making lifestyle changes and ICS services. Volunteers reported the need for more training and improvements to some organisational aspects. More rigorous cancer health promotion programmes are required to counter disrupted cancer services. Our results suggest such cancer roadshow events are both feasible and beneficial at this time post pandemic. Key messages • Health literacy is continuous process to achieve positive health outcomes. • Reaching out to people in different setting is acceptable and potentially effective.

Targeting non-structural proteins and 3CL(pro) in SARS-CoV-2 virus using phytochemicals from medicinal plants- In-silico approach

In the present study, the main protease 3CL(pro) and non-structural protein (NSP-12 with co-factors 7 and 8) trimer complex are used to study the protein-drug interactions with the phytochemicals from Ocimum Sanctum, Tinospora Cordifolia, Glycyrrhiza Glabra, and Azadirachta Indica. Which can give insight to be used as potent antiviral drugs against SARS-CoV-2. Twenty phytochemicals, five from each plant species, known for their wide range of biological activities were chosen from the literature. The in-silico study was carried out using virtual screening tools and the top five, which showed the least binding energies, were selected. Molecular docking tools revealed that gedunin and epoxy azadiradione proved to be excellent inhibitors for 3CL(pro) and so did Tinosporide for nonstructural-protein complex. Further, the best-hit phytochemicals with respect to structure similarities with FDA drugs and investigatory drugs, were considered for comparative study. Molecular docking was done to check the drug-protein interactions and to check the inhibitory responses of these drugs against the viral protein. The analyses showed that the phytochemicals had similar responses on the protein complex but with exceptionally higher inhibitory responses hence which may be taken for further clinical study.

Physician Health in the Times of COVID-19.

BACKGROUND AND OBJECTIVES: A virtual registry study evaluating real world evidence on physicians' use of prophylactic regimens for protection against SARS-CoV-2. This paper summarizes the interim results. METHODS: Asymptomatic physicians at risk of acquiring SARS-CoV-2 responded to online questions at baseline and 7 weeks post-baseline. Baseline data included demographics, prophylaxis regimen (including "no prophylaxis") and start date. Participants who provided complete week-7 data (information on type of health facility [COVID/Non-COVID], number of presumed/confirmed cases exposed to, PPE use, SARS-CoV-2 testing and symptoms, regimen adherence and intercurrent illness) comprised the Completer population. Limited data (regimen adherence, SARS-CoV-2 testing) was collected for participants who failed to provide complete week7 data. Those providing limited/complete information comprised the Evaluable population. RESULTS: Of 369 enrolled participants, 182 (mean age 42±11.05 years) comprised the Evaluable population. They showed a male preponderance (67.6%). Practitioners from Maharashtra (59.9%) and specialties of Pediatrics, Internal Medicine, Anesthesiology and Critical Care (63.2%) accounted for the majority. ICMR's HCQ prophylaxis regimen was initiated by 125 (68.7%) participants with 31 (17%) initiating 'No prophylaxis'. The highest adherence was for the ICMRregimen (87.2%). In the Completer population comprising 150 participants, 87 were exposed to presumed (81) and/or confirmed cases (60). Most exposures to confirmed cases (49, 81.7%) were high-risk. PPE use was generally high (75-100%). Most participants (94.7%) did not report an AE. The proportions with an AE was similar with ICMR regimen (5.9%) and no prophylaxis (6.5%). INTERPRETATION AND CONCLUSIONS: Physicians in India preferred ICMR's HCQ regimen. The regimen appears to be safe and associated with a high level of adherence.

Principles and applications of infrared thermal imaging

Nowadays, due to the increasing spread of the coronavirus which effects the human temperature, there is a need for a framework useful for identifying temperature without any interaction with the human body. To measure the temperature in the human body in a noncontact way, infrared imaging technology can be used for the measurement of the body temperature quickly and effectively. The thermal imaging is a noncontact way of measuring the temperature which uses infrared radiation as a temperature-dependent parameter. Using thermal imaging, humans and other homothetic animals become visible even in day or night, so it is helpful for the military applications as well. A review of the other various applications of the infrared thermal imaging and the fundamentals of thermal imaging has been discussed in this paper. The further useful application of thermal cameras for COVID-19 is also analyzed. ©2020 by Begell House, Inc.